PATHOS is a web facility that allows one to display metabolites identified by mass spectrometry in the context of the metabolic pathways in which they occur. Textual output provides a listing of areas of metabolism, with the option of generating a Kegg map on which identified metabolites are marked. Input data may consist of either experimental M/z values from mass spectrometrometric peaks, or refined lists of metabolites generated from the mass spec. data by other means.
Quantitative data for the abundance of metabolites in different experimental conditions may be included in the input file. In this case changes are indicated by colour-coding, and clicking on metabolites on the maps generates appropriate bar charts. New users should consult the on-line Instructions (menu bar, above) or the Instruction Manual, and may wish to download the example files provided (links, below).
Technical information about Pathos can be found in the publication: Leader, D.P., Burgess, K., Creek, D. and Barrett, M.P. (2011) Rapid Commun. Mass Spectrom., 25, 3422–3426.
Instruction manualExample files
Select and Upload File
Select a suitable data file and then click ‘Upload’.